Cyclosporine A, CsA ELISA Kit
RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ
THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Ciclosporin,Cyclosporine A, Ciclosporin,cyclosporine,cyclosporin,cyclosporin
Human Ciclosporin ELISA Kit, Human Cyclosporine A ELISA Kit, Human cyclosporine
ELISA Kit, Human cyclosporin ELISA Kit, Human CSA ELISA Kit
This immunoassay kit allows for the in vitro quantitative determination
of Human ciclosporin,Ciclosporin concentrations in serum, plasma,
tissue homogenates, cell culture
supernates, and other biological fluids.
The ELISA is based on the competitive binding
enzyme immunoassay technique. The microtiter plate provided in this
kit has been pre-coated with an antibody specific to Ciclosporin, During the reaction, Ciclosporin in the sample or standard competes with a fixed
amount of biotin-labeled Ciclosporin for sites on a
antibody specific to Ciclosporin. Excess conjugate and unbound sample or standard are
washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm ± 2 nm. The concentration of Ciclosporin in the samples is then determined by comparing the
O.D. of the samples to the standard curve.
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This product was added to our catalog on Tuesday 12 June, 2012.