RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ
THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
hemoglobin A1c, HbA1c
Glycosylated hemoglobin A1c ELISA Kit,Rat HbA1c ELISA Kit,Glycosylated
hemoglobin A1c ELISA Kit,HbA1c ELISA Kit,ELISA Kit, Glycosylated hemoglobin
This immunoassay kit allows for the in vitro quantitative determination of rat Glycated hemoglobin A1c concentrations in serum, Plasma, Urine,tissue homogenates
culture supernates and Other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycated hemoglobin A1c. Standards or samples are then added to the appropriate microtiter
plate wells with a biotin-conjugated antibody preparation specific for Glycated
hemoglobin A1c and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that
contain Glycated hemoglobin A1c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycated
hemoglobin A1c in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
This product was added to our catalog on Tuesday 12 June, 2012.