RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ
THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Sonic hedgehog protein(SHH),HHG-1, Shh,HHG1;
HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; sonic hedgehog
protein; sonic hedgehog
This immunoassay kit allows for the in vitro quantitative determination of rat sonic hedgehog
protein,SHH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other
The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHH. Standards or samples are then added to the appropriate microtiter
plate wells with a biotin-conjugated polyclonal antibody preparation specific
for SHH and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. Only those
wells that contain SHH,
biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically
at a wavelength of 450 nm ± 2 nm. The concentration of SHH in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
PDF manual download
This product was added to our catalog on Tuesday 12 June, 2012.