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Rat Sonic hedgehog protein, SHH ELISA Kit

$756.00

Rat Sonic hedgehog protein, SHH ELISA Kit

Catalog No: E1831r

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Sonic hedgehog protein(SHH),HHG-1, Shh,HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog homolog; sonic hedgehog protein; sonic hedgehog

 

Search name

Rat Sonic hedgehog protein ELISA Kit, Rat SHH ELISA Kit, Rat HHG-1 ELISA Kit, Rat HHG1 ELISA Kit, Rat HLP3 ELISA Kit, Rat HPE3 ELISA Kit, Rat MCOPCB5 ELISA Kit, Rat SMMCI ELISA Kit, Rat TPT ELISA Kit, Rat TPTPS ELISA Kit, Rat sonic hedgehog homolog ELISA Kit, Rat sonic hedgehog ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat sonic hedgehog protein,SHH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SHH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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  • Model: E1831r
  • 20 Units in Stock



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This product was added to our catalog on Tuesday 12 June, 2012.