ATCC15834 Agrobacterium tumefaciens (Rhizogenes) Strain

What is Agrobacterium tumefaciens ATCC15834? ATCC15834 is a wild-type strain of Agrobacterium tumefaciens (reclassified as Agrobacterium rhizogenes) derived from the American Type Culture Collection (ATCC). It carries the pRi15834 Ri (root-inducing) plasmid of the agropine type, which enables the bacterium to infect plant tissues and induce genetically stable hairy root formation across a remarkably broad host range.

Ri Plasmid & Hairy Root Transformation: The pRi15834 agropine-type Ri plasmid transfers T-DNA from the right border (RB) region into the plant genome, triggering the proliferation of adventitious roots at infection sites. These transformed "hairy roots" exhibit rapid hormone-independent growth, genetic stability, and elevated secondary metabolite production — making them invaluable platforms for producing high-value phytochemicals that are difficult or uneconomical to synthesize chemically or extract from whole plants.

Primary Applications:

• Hairy Root Culture Systems: Induction of transgenic hairy roots in dicotyledonous and monocotyledonous plants for sustained secondary metabolite biosynthesis
• Medicinal Plant Biotechnology: Production of paclitaxel (Taxol), artemisinin, ginsenosides, and other pharmaceutical compounds via transformed root cultures
• Plant Genetic Engineering Research: Model system for studying Agrobacterium-mediated gene transfer, T-DNA integration mechanisms, and opine metabolism
• Functional Genomics: Gene function validation through hairy root-based overexpression or RNAi knockdown in diverse plant species
• Host Range Validation: Demonstrated infectivity across Poaceae (grasses), Fabaceae (legumes), Solanaceae (tobacco), and various gymnosperms

1. This product is FOR RESEARCH USE ONLY!
2. This product is glycerol packed. Please culture it as soon as possible after receipt, then harvest and store at –80°C.
3. Shipping temperature is 2–8°C. Upon receipt, transfer immediately to –80°C storage if not culturing immediately.
4. ATCC15834 grows slowly. Transformed bacteria on antibiotic-containing plates require up to 72 hours of incubation for visible colony formation.
5. Please use the active culture as soon as possible; strains are not recommended for long-term storage in active culture form.
6. If other culture mediums or growth conditions are used, our warranty for viability is no longer valid.