Bovine Acetylcholine receptor subunit epsilon, CHRNE ELISA KIT
Product Name:Bovine Acetylcholine receptor subunit epsilon, CHRNE ELISA KIT
Packing:96T
Catalog No.:ELI-24654b
Gene Name:Bovine Acetylcholine receptor subunit epsilon, CHRNE
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Acetylcholine receptor subunit epsilon, CHRNE
Alternative Name:CHRNE,Bovine Acetylcholine receptor subunit epsilon, CHRNE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Acetylcholine receptor subunit epsilon, CHRNE ELISA KIT allows for the in vitro quantitative determination of Bovine Acetylcholine receptor subunit epsilon, CHRNE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Acetylcholine receptor subunit epsilon, CHRNE ELISA KIT has been pre-coated with an Bovine Acetylcholine receptor subunit epsilon, CHRNE antibody specific to Bovine Acetylcholine receptor subunit epsilon, CHRNE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Acetylcholine receptor subunit epsilon, CHRNE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Acetylcholine receptor subunit epsilon, CHRNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Acetylcholine receptor subunit epsilon, CHRNE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.
