Bovine 14- 3- 3 protein eta, YWHAH ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Protein AS1,YWHAH,14 3 3 protein eta,YWHA1; 14-3-3 eta; 14-3-3 protein eta; tyrosine 3-monooxygenase activation protein, eta polypeptide,tryptophan 5-monooxygenase activation protein eta polypeptide

Search name

Bovine Protein AS1 ELISA KIT ,Bovine YWHAH ELISA KIT ,Bovine 14 3 3 protein eta ELISA KIT ,Bovine YWHA1 ELISA KIT ,Bovine 14-3-3 eta ELISA KIT ,Bovine 14-3-3 protein eta ELISA KIT ,Bovine tyrosine 3-monooxygenase activation protein eta polypeptide ELISA KIT ,Bovine tryptophan 5-monooxygenase activation protein eta polypeptide ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine 14- 3- 3 protein eta, YWHAH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAH, During the reaction, YWHAH in the sample or standard competes with a fixed amount of biotin-labeled YWHAH for sites on a pre-coated Monoclonal antibody specific to YWHAH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of YWHAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized Product