Bovine Acetoacetic acid ELISA Kit

Product Name:Bovine Acetoacetic acid ELISA Kit
Packing:96T

Catalog No.:ELA-E0986b

Gene Name:Bovine Acetoacetic acid

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Bovine Acetoacetic acid

Alternative Name:ACAC,Bovine Acetoacetic acid
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Acetoacetic acid ELISA Kit allows for the in vitro quantitative determination of Bovine Acetoacetic acid concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Acetoacetic acid ELISA Kit has been pre-coated with an Bovine Acetoacetic acid antibody specific to Bovine Acetoacetic acid .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Acetoacetic acid and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Acetoacetic acid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Acetoacetic acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!