Bovine Angiotensin 1, AGT1 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Angiotensin-1,AGT,ANHU, SERPINA8, alpha-1 antiproteinase, antitrypsin, angiotensin I, angiotensin II, angiotensinogen,
pre-angiotensinogen, serine (or cysteine) proteinase inhibitor, serpin A8, angiotensinogen,serpin peptidase inhibitor clade A member 8,
cysteine proteinase inhibitor

Search name
Bovine Angiotensin-1 ELISA KIT ,Bovine AGT ELISA KIT ,Bovine ANHU ELISA KIT ,Bovine SERPINA8 ELISA KIT ,Bovine alpha-1
antiproteinase ELISA KIT ,Bovine antitrypsin ELISA KIT ,Bovine angiotensin I ELISA KIT ,Bovine angiotensin II ELISA
KIT ,Bovine angiotensinogen ELISA KIT ,Bovine pre-angiotensinogen ELISA KIT ,Bovine serine (or cysteine) proteinase
inhibitor ELISA KIT ,Bovine serpin A8 ELISA KIT ,Bovine angiotensinogen ELISA KIT ,Bovine serpin peptidase inhibitor clade A
member 8 ELISA KIT ,Bovine cysteine proteinase inhibitor ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Angiotensin-1 concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Angiotensin-1, During the reaction, Angiotensin-1 in the sample or standard competes with a fixed
amount of biotin-labeled Angiotensin-1 for sites on a pre-coated Monoclonal antibody specific to Angiotensin-1. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Angiotensin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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