Bovine AP- 3 complex subunit beta- 1, AP3B1 ELISA KIT
Product Name:Bovine AP- 3 complex subunit beta- 1, AP3B1 ELISA KIT
Packing:96T
Catalog No.:ELI-49233b
Gene Name:Bovine AP-3 complex subunit beta-1, AP3B1
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine AP-3 complex subunit beta-1, AP3B1
Alternative Name:AP3B1,Bovine AP-3 complex subunit beta-1, AP3B1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine AP- 3 complex subunit beta- 1, AP3B1 ELISA KIT allows for the in vitro quantitative determination of Bovine AP-3 complex subunit beta-1, AP3B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine AP- 3 complex subunit beta- 1, AP3B1 ELISA KIT has been pre-coated with an Bovine AP-3 complex subunit beta-1, AP3B1 antibody specific to Bovine AP-3 complex subunit beta-1, AP3B1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine AP-3 complex subunit beta-1, AP3B1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine AP-3 complex subunit beta-1, AP3B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine AP-3 complex subunit beta-1, AP3B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!