Bovine Apolipoprotein C- III, APOC3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

APOC3,APOCIII; HALP2; apo-CIII; apoC-III; apolipoprotein C3; apolipoprotein C-III

Search name

Bovine APOC3 ELISA KIT ,Bovine APOCIII ELISA KIT ,Bovine HALP2 ELISA KIT ,Bovine apo-CIII ELISA KIT ,Bovine apoC-III ELISA KIT ,Bovine apolipoprotein C3 ELISA KIT ,Bovine apolipoprotein C-III ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine apolipoprotein c-iii,Apo-CIII concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apo-CIII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo-CIII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo-CIII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo-CIII in the samples is then determined by comparing the O.D. of the samples to the standard curve.