Bovine Aurora kinase A, AURKA ELISA KIT
Product Name:Bovine Aurora kinase A, AURKA ELISA KIT
Packing:96T
Catalog No.:ELI-50023b
Gene Name:Bovine Aurora kinase A, AURKA
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Aurora kinase A, AURKA
Alternative Name:AURKA,Bovine Aurora kinase A, AURKA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Aurora kinase A, AURKA ELISA KIT allows for the in vitro quantitative determination of Bovine Aurora kinase A, AURKA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Aurora kinase A, AURKA ELISA KIT has been pre-coated with an Bovine Aurora kinase A, AURKA antibody specific to Bovine Aurora kinase A, AURKA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Aurora kinase A, AURKA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Aurora kinase A, AURKA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Aurora kinase A, AURKA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!