Bovine basic fibroblast growth factor ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Bovine BFGF ELISA KIT ,Bovine basic fibroblast growth factor ELISA KIT ,Bovine FGF-2 ELISA KIT ,Bovine FGF2 ELISA KIT ,Bovine basic fibroblast growth factor bFGF ELISA KIT ,Bovine FGFB ELISA KIT ,Bovine HBGF-2 ELISA KIT ,Bovine fibroblast growth factor 2 (basic) ELISA KIT ,Bovine heparin-binding growth factor 2 ELISA KIT ,Bovine prostatropin ELISA KIT ,Bovine fibroblast growth factor 2 ELISA KIT ,Bovine  Heparin-binding growth factor 2 ELISA KIT ,Bovine HBGF-2 ELISA KIT

Intended use

Bovine BFGF ELISA KIT allows for the in vitro quantitative determination of Bovine heparin-binding growth factor 2,HBGF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Bovine BFGF ELISA KIT has been pre-coated with an antibody specific to HBGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HBGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HBGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HBGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine BFGF ELISA KIT Manual Request

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