Bovine Beta- centractin, ACTR1B ELISA KIT
Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  ACTR1B

Protein Name  ARP1 actin-related protein 1 homolog B, centractin beta

Alternative Name

Bovine ACTR1B ELISA Kit ,Bovine ARP1B ELISA Kit ,Bovine CTRN2 ELISA Kit ,Bovine PC3 ELISA Kit ,Bovine beta-centractin ELISA Kit ,Bovine ARP1 actin-related protein 1 homolog B centractin beta ELISA Kit ,Bovine actin-related protein 1B ELISA Kit ,Bovine centractin beta ELISA Kit ,Bovine ARP1 actin related protein 1 homolog B ELISA Kit
 

Intended use

Bovine ACTR1B ELISA KIT allows for the in vitro quantitative determination of ACTR1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Bovine ACTR1B ELISA KIT has been pre-coated with an ACTR1B antibody specific to ACTR1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ACTR1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACTR1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTR1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20