Bovine Beta- Hydroxybutyric Acid ELISA Kit
Product Name:Bovine Beta- Hydroxybutyric Acid ELISA Kit
Gene Name:Bovine Beta-Hydroxybutyric Acid
Target Protein Name:Bovine Beta-Hydroxybutyric Acid
Alternative Name:Beta-OHB,Bovine Beta-Hydroxybutyric Acid
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Beta- Hydroxybutyric Acid ELISA Kit allows for the in vitro quantitative determination of Bovine Beta-Hydroxybutyric Acid concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Beta- Hydroxybutyric Acid ELISA Kit has been pre-coated with an Bovine Beta-Hydroxybutyric Acid antibody specific to Bovine Beta-Hydroxybutyric Acid .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Beta-Hydroxybutyric Acid and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Beta-Hydroxybutyric Acid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Beta-Hydroxybutyric Acid in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!