Bovine BolA- like protein 3, Bola3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
BOLA3,MMDS2; bolA homolog 3; bolA-like protein 3; bolA family member 3
Search name
Bovine BOLA3 ELISA KIT ,Bovine MMDS2 ELISA KIT ,Bovine bolA homolog 3 ELISA KIT ,Bovine bolA-like protein 3 ELISA KIT ,Bovine bolA family member 3 ELISA KIT
Intended use
Bovine BOLA3 ELISA KIT allows for the in vitro quantitative determination of Bovine BolA- like protein 3, Bola3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine BOLA3 ELISA KIT has been pre-coated with an antibody specific to Bola3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bola3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bola3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bola3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine BOLA3 ELISA KIT Range 1.5-100ng/ml
BOLA3 Related Products
> BOLA3 ELISA KITS
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