Bovine C- reactive protein ELISA Kit
Product Name:Bovine C- reactive protein ELISA Kit
Packing:96T
Catalog No.:ELA-E0829b
Gene Name:Bovine C-reactive protein
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine C-reactive protein
Alternative Name:CRP,Bovine C-reactive protein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine C- reactive protein ELISA Kit allows for the in vitro quantitative determination of Bovine C-reactive protein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine C- reactive protein ELISA Kit has been pre-coated with an Bovine C-reactive protein antibody specific to Bovine C-reactive protein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine C-reactive protein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine C-reactive protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine C-reactive protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!