Bovine Calcitriol ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Calcitriol,1,25-dihydroxycholecalciferol,1,25-dihydroxyvitamin D3,DVD,DHVD 3

Search name

Bovine Calcitriol ELISA Kit, Bovine 1,25-dihydroxycholecalciferol ELISA Kit, Bovine 1,25-dihydroxyvitamin D3 ELISA Kit, Bovine DVD ELISA Kit, Bovine DHVD 3 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Calcitriol concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcitriol, During the reaction, Calcitriol in the sample or standard competes with a fixed amount of biotin-labeled Calcitriol for sites on a pre-coated Monoclonal antibody specific to Calcitriol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Calcitriol in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF manual download

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