Bovine Calf intestinal alkaline phosphatase ELISA Kit
Product Name:Bovine Calf intestinal alkaline phosphatase ELISA Kit
Packing:96T
Catalog No.:ELA-E0233b
Gene Name:Bovine Calf intestinal alkaline phosphatase
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Calf intestinal alkaline phosphatase
Alternative Name:CIAP,Bovine Calf intestinal alkaline phosphatase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Calf intestinal alkaline phosphatase ELISA Kit allows for the in vitro quantitative determination of Bovine Calf intestinal alkaline phosphatase concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Calf intestinal alkaline phosphatase ELISA Kit has been pre-coated with an Bovine Calf intestinal alkaline phosphatase antibody specific to Bovine Calf intestinal alkaline phosphatase .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Calf intestinal alkaline phosphatase and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Calf intestinal alkaline phosphatase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Calf intestinal alkaline phosphatase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!