Bovine Carbonic anhydrase 4, CA4 ELISA KIT

Product Name:Bovine Carbonic anhydrase 4, CA4 ELISA KIT
Packing:96T

Catalog No.:ELI-03640b

Gene Name:Bovine Carbonic anhydrase 4, CA4

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Carbonic anhydrase 4, CA4

Alternative Name:CA4,Bovine Carbonic anhydrase 4, CA4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Carbonic anhydrase 4, CA4 ELISA KIT allows for the in vitro quantitative determination of Bovine Carbonic anhydrase 4, CA4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Carbonic anhydrase 4, CA4 ELISA KIT has been pre-coated with an Bovine Carbonic anhydrase 4, CA4 antibody specific to Bovine Carbonic anhydrase 4, CA4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Carbonic anhydrase 4, CA4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Carbonic anhydrase 4, CA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Carbonic anhydrase 4, CA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!