Bovine Carboxylesterase 4A, CES4A ELISA KIT
Product Name:Bovine Carboxylesterase 4A, CES4A ELISA KIT
Packing:96T
Catalog No.:ELI-09958b
Gene Name:Bovine Carboxylesterase 4A, CES4A
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Carboxylesterase 4A, CES4A
Alternative Name:CES4A,Bovine Carboxylesterase 4A, CES4A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Carboxylesterase 4A, CES4A ELISA KIT allows for the in vitro quantitative determination of Bovine Carboxylesterase 4A, CES4A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Carboxylesterase 4A, CES4A ELISA KIT has been pre-coated with an Bovine Carboxylesterase 4A, CES4A antibody specific to Bovine Carboxylesterase 4A, CES4A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Carboxylesterase 4A, CES4A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Carboxylesterase 4A, CES4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Carboxylesterase 4A, CES4A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!