Bovine Cholinesterase, BCHE ELISA KIT

96 Tests
Operating instruction
FOR RESEARCH USE ONLY!

Search name
Bovine BChE ELISA KIT ,Bovine HGNC ELISA KIT ,Bovine BuChE ELISA KIT ,Bovine BCHE ELISA KIT ,Bovine Cholinesterase ELISA KIT, Bovine pseudocholinesterase ELISA KIT ,Bovine plasma cholinesterase ELISA KIT ,Bovine CHE1 ELISA KIT ,Bovine CHE2 ELISA KIT ,Bovine acylcholine acylhydrolase ELISA KIT ,Bovine butyrylcholine esterase ELISA KIT ,Bovine choline esterase II ELISA KIT ,Bovine cholinesterase (serum) 2 ELISA KIT ,Bovine cholinesterase 1 ELISA KIT ,Bovine pseudocholinesterase ELISA KIT ,Bovine Butyrylcholinesterase ELISA KIT

Intended use
Bovine BChE ELISA KIT allows for the in vitro quantitative determination of bovine Cholinesterase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in Bovine BChE ELISA KIT has been pre-coated with an antibody specific to Cholinesterase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cholinesterase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cholinesterase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cholinesterase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 31.2-2000pg/ml

Bovine HGNC ELISA KIT PDF Manual download

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