Bovine Claudin- 4, CLDN4 ELISA KIT

Product Name:Bovine Claudin- 4, CLDN4 ELISA KIT
Packing:96T

Catalog No.:ELI-46804b

Gene Name:Bovine Claudin-4, CLDN4

Detect Range:3.125-200ng/ml

Sensitivity:1.875ng/ml

Target Protein Name:Bovine Claudin-4, CLDN4

Alternative Name:CLDN4,Bovine Claudin-4, CLDN4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Claudin- 4, CLDN4 ELISA KIT allows for the in vitro quantitative determination of Bovine Claudin-4, CLDN4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Claudin- 4, CLDN4 ELISA KIT has been pre-coated with an Bovine Claudin-4, CLDN4 antibody specific to Bovine Claudin-4, CLDN4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Claudin-4, CLDN4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Claudin-4, CLDN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Claudin-4, CLDN4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!