Bovine Collagen alpha- 1(II) chain, COL2A1 ELISA KIT
Product Name:Bovine Collagen alpha- 1(II) chain, COL2A1 ELISA KIT
Packing:96T
Catalog No.:ELI-02038b
Gene Name:Bovine Collagen alpha-1(II) chain, COL2A1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Collagen alpha-1(II) chain, COL2A1
Alternative Name:COL2A1,Bovine Collagen alpha-1(II) chain, COL2A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Collagen alpha- 1(II) chain, COL2A1 ELISA KIT allows for the in vitro quantitative determination of Bovine Collagen alpha-1(II) chain, COL2A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Collagen alpha- 1(II) chain, COL2A1 ELISA KIT has been pre-coated with an Bovine Collagen alpha-1(II) chain, COL2A1 antibody specific to Bovine Collagen alpha-1(II) chain, COL2A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Collagen alpha-1(II) chain, COL2A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Collagen alpha-1(II) chain, COL2A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Collagen alpha-1(II) chain, COL2A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!