Bovine Cyclic Diguanylate, Cdi GMP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Cyclic Diguanylate,CdiGMP; c-di-GMP
Search name
Bovine CdiGMP ELISA Kit,Bovine Cyclic Diguanylate ELISA Kit, Cyclic Diguanylate ELISA Kit,CdiGMP ELISA Kit
Intended use
Bovine CdiGMP ELISA Kit allows for the in vitro quantitative determination of general Cyclic Diguanylate,CdiGMP concentrations in serum,plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Bovine CdiGMP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to CdiGMP, During the reaction, CdiGMP in the sample or standard competes with a fixed amount of biotin-labeled CdiGMP for sites on a pre-coated Monoclonal antibody specific to CdiGMP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
CdiGMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine CdiGMP ELISA Kit Range 0.78-50ng/ml
Bovine CdiGMP ELISA Kit PDF manual download
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