Bovine Cyclic guanosine monophosphate, cGMP ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Cyclic guanosine monophosphate,;

Search name
Bovine Cyclic guanosine monophosphate ELISA Kit, Bovine cGMP ELISA Kit, Cyclic guanosine monophosphate ELISA Kit,
cGMP ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of cyclic guanosine monophosphate,Cyclic guanosine
monophosphate concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Cyclic guanosine monophosphate, During the reaction, Cyclic guanosine monophosphate in the
sample or standard competes with a fixed amount of biotin-labeled Cyclic guanosine monophosphate for sites on a pre-coated
Monoclonal antibody specific to Cyclic guanosine monophosphate. Excess conjugate and unbound sample or standard are washed from
the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyclic guanosine
monophosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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