Bovine Dehydroepiandrosterone, DHEA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Dehydroepiandrosterone,Dehydroepiandrosterone,DHEA, androstenolone, prasterone, 3β-hydroxyandrost-5-en-17-one,
5-androsten-3β-ol-17-one

Search name
Bovine Dehydroepiandrosterone, DHEA ELISA Kit, Bovine DHEA ELISA Kit, Bovine androstenolone ELISA Kit, Bovine
prasterone ELISA Kit, Bovine 3β-hydroxyandrost-5-en-17-one ELISA Kit, Bovine 5-androsten-3β-ol-17-one ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of dehydroepiandrosterone,Dehydroepiandrosterone
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Dehydroepiandrosterone, During the reaction, Dehydroepiandrosterone in the sample or standard
competes with a fixed amount of biotin-labeled Dehydroepiandrosterone for sites on a pre-coated Monoclonal antibody specific to
Dehydroepiandrosterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dehydroepiandrosterone in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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