Bovine Dopamine ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Dopamine,DA, 4-(2-aminoethyl)benzene-1,2-diol,2-(3,4-dihydroxyphenyl)ethylamine; 3,4-dihydroxyphenethylamine; 3-hydroxytyramine;
DA; Intropin; Revivan; Oxytyramine

Search name
Bovine Dopamine ELISA Kit, Bovine 4-(2-aminoethyl)benzene-1,2-diol ELISA Kit, Bovine
2-diol,2-(3,4-dihydroxyphenyl)ethylamine ELISA Kit, Bovine 3,4-dihydroxyphenethylamine ELISA Kit, Bovine 3-hydroxytyramine
ELISA Kit, Bovine DA ELISA Kit,Bovine Intropin ELISA Kit, Bovine Revivan ELISA Kit, Bovine Oxytyramine ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro Quantitative determination of Bovine dopamine,Dopamine concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay techniQue. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Dopamine, During the reaction, Dopamine in the sample or standard competes with a fixed
amount of biotin-labeled Dopamine for sites on a pre-coated Monoclonal antibody specific to Dopamine. Excess conjugate and unbound
sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration
of Dopamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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