Bovine UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DPAGT1,ALG7; CDG-Ij; CDG1J; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT; GlcNAc-1-P transferase; N-acetylglucosamine-1-phosphate transferase; UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra; dolichyl-phosphate alpha-N-acetylglucosaminyltransferase; dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)

Search name

Bovine DPAGT1 ELISA KIT ,Bovine ALG7 ELISA KIT ,Bovine CDG-Ij ELISA KIT ,Bovine CDG1J ELISA KIT ,Bovine CMSTA2 ELISA KIT ,Bovine D11S366 ELISA KIT ,Bovine DGPT ELISA KIT ,Bovine DPAGT ELISA KIT ,Bovine DPAGT2 ELISA KIT ,Bovine G1PT ELISA KIT ,Bovine GPT ELISA KIT ,Bovine UAGT ELISA KIT ,Bovine UGAT ELISA KIT ,Bovine GlcNAc-1-P transferase ELISA KIT ,Bovine N-acetylglucosamine-1-phosphate transferase ELISA KIT ,Bovine UDP-GlcNAc:dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Bovine UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase ELISA KIT ,Bovine dolichyl-phosphate ELISA KIT ,Bovine UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P tra ELISA KIT ,Bovine dolichyl-phosphate alpha-N-acetylglucosaminyltransferase ELISA KIT ,Bovine dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine UDP- N- acetylglucosamine- - dolichyl- phosphate N- acetylglucosaminephosphotransferase, DPAGT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPAGT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DPAGT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DPAGT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPAGT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.