Bovine E3 ubiquitin- protein ligase RNF181, RNF181 ELISA KIT

Product Name:Bovine E3 ubiquitin- protein ligase RNF181, RNF181 ELISA KIT
Packing:96T

Catalog No.:ELI-44962b

Gene Name:Bovine E3 ubiquitin-protein ligase RNF181, RNF181

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Bovine E3 ubiquitin-protein ligase RNF181, RNF181

Alternative Name:RNF181,Bovine E3 ubiquitin-protein ligase RNF181, RNF181
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine E3 ubiquitin- protein ligase RNF181, RNF181 ELISA KIT allows for the in vitro quantitative determination of Bovine E3 ubiquitin-protein ligase RNF181, RNF181 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine E3 ubiquitin- protein ligase RNF181, RNF181 ELISA KIT has been pre-coated with an Bovine E3 ubiquitin-protein ligase RNF181, RNF181 antibody specific to Bovine E3 ubiquitin-protein ligase RNF181, RNF181 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine E3 ubiquitin-protein ligase RNF181, RNF181 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine E3 ubiquitin-protein ligase RNF181, RNF181, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine E3 ubiquitin-protein ligase RNF181, RNF181 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!