Bovine Early growth response protein 1, EGR1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!

PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Egr-1,AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225; nerve growth factor-induced protein A; transcription factor ETR103; transcription factor Zif268; zinc finger protein 225; zinc finger protein Krox-24; early growth response 1,Early growth response protein 1(EGR-1),; Zinc finger protein Krox-24,Zinc finger protein 225,Transcription factor Zif268,Transcription factor ETR103,AT225,Nerve growth factor-induced protein A(NGFI-A),;

Search name

Bovine Egr-1 ELISA KIT ,Bovine AT225 ELISA KIT ,Bovine G0S30 ELISA KIT ,Bovine KROX-24 ELISA KIT ,Bovine NGFI-A ELISA KIT ,Bovine TIS8 ELISA KIT ,Bovine ZIF-268 ELISA KIT ,Bovine ZNF225 ELISA KIT ,Bovine nerve growth factor-induced protein A ELISA KIT ,Bovine transcription factor ETR103 ELISA KIT ,Bovine transcription factor Zif268 ELISA KIT ,Bovine zinc finger protein 225 ELISA KIT ,Bovine zinc finger protein Krox-24 ELISA KIT ,Bovine early growth response 1 ELISA KIT ,Bovine Early growth response protein 1 ELISA KIT ,Bovine EGR-1 ELISA KIT ,Bovine Zinc finger protein Krox-24 ELISA KIT ,Bovine Zinc finger protein 225 ELISA KIT ,Bovine Transcription factor Zif268 ELISA KIT ,Bovine Transcription factor ETR103 ELISA KIT ,Bovine AT225 ELISA KIT ,Bovine Nerve growth factor-induced protein A ELISA KIT ,Bovine NGFI-A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine early growth response protein 1,EGR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EGR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EGR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EGR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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