Bovine Fructosamine, FA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!

Alternative name
Bovine Fructosamine ELISA Kit, Bovine FA ELISA Kit,Fructosamine ELISA Kit,FA ELISA Kit,ELISA Kit, Fructosamine,FA

Intended use
Bovine Fructosamine ELISA Kit allows for the in vitro quantitative determination of Bovine fructosamine,FA concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Fructosamine, During the reaction, Fructosamine in the sample or standard competes with a fixed
amount of biotin-labeled Fructosamine for sites on a pre-coated Monoclonal antibody specific to Fructosamine. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Fructosamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 15.6-1000 umol/l

PDF manual download

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