Bovine Galactosylceramide sulfotransferase, GAL3ST1 ELISA KIT (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Bovine GAL3ST1 ELISA KIT ,Bovine CST ELISA KIT , Bovine cerebroside sulfotransferase ELISA KIT , Bovine 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase ELISA KIT , Bovine galactose-3-O-sulfotransferase 1 ELISA KIT

Intended use

Bovine GAL3ST1 ELISA KIT allows for the in vitro quantitative determination of Bovine Galactosylceramide sulfotransferase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Bovine GAL3ST1 ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAL3ST1, During the reaction, GAL3ST1 in the sample or standard competes with a fixed amount of biotin-labeled GAL3ST1 for sites on a pre-coated Monoclonal antibody specific to GAL3ST1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GAL3ST1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Customized product    Min.Order 5kits

Delivery time 4-6month

Order Bovine GAL3ST1 ELISA kit by email:[email protected]