Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT

Product Name:Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT
Packing:96T

Catalog No.:ELI-02890b

Gene Name:Bovine Glycogen phosphorylase, muscle form, PYGM

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine Glycogen phosphorylase, muscle form, PYGM

Alternative Name:PYGM,Bovine Glycogen phosphorylase, muscle form, PYGM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT allows for the in vitro quantitative determination of Bovine Glycogen phosphorylase, muscle form, PYGM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Glycogen phosphorylase, muscle form, PYGM ELISA KIT has been pre-coated with an Bovine Glycogen phosphorylase, muscle form, PYGM antibody specific to Bovine Glycogen phosphorylase, muscle form, PYGM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Glycogen phosphorylase, muscle form, PYGM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Glycogen phosphorylase, muscle form, PYGM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Glycogen phosphorylase, muscle form, PYGM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!