Bovine GTP- binding protein Rheb, RHEB ELISA KIT
Product Name:Bovine GTP- binding protein Rheb, RHEB ELISA KIT
Packing:96T
Catalog No.:ELI-52257b
Gene Name:Bovine GTP-binding protein Rheb, RHEB
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine GTP-binding protein Rheb, RHEB
Alternative Name:RHEB,Bovine GTP-binding protein Rheb, RHEB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine GTP- binding protein Rheb, RHEB ELISA KIT allows for the in vitro quantitative determination of Bovine GTP-binding protein Rheb, RHEB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine GTP- binding protein Rheb, RHEB ELISA KIT has been pre-coated with an Bovine GTP-binding protein Rheb, RHEB antibody specific to Bovine GTP-binding protein Rheb, RHEB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine GTP-binding protein Rheb, RHEB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine GTP-binding protein Rheb, RHEB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine GTP-binding protein Rheb, RHEB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!