Bovine HBS1L ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  HBS1L

Protein Name  HBS1-like protein

Alternative Name

HBS1L,HBS1,EF-1a; ERFS; HSPC276; eRF3c; HBS1-like protein; ERF3-similar protein; Hsp70 subfamily B suppressor 1-like protein; eRF3 family member; HBS1 like translational GTPase

Intended use

Bovine HBS1L ELISA KIT allows for the in vitro quantitative determination of HBS1L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Bovine HBS1L ELISA KIT has been pre-coated with an HBS1L antibody specific to HBS1L. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HBS1L and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HBS1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HBS1L in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20