Bovine Heat shock 70 kDa protein 13, HSPA13 ELISA KIT
Product Name:Bovine Heat shock 70 kDa protein 13, HSPA13 ELISA KIT
Packing:96T
Catalog No.:ELI-20851b
Gene Name:Bovine Heat shock 70 kDa protein 13, HSPA13
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Heat shock 70 kDa protein 13, HSPA13
Alternative Name:HSPA13,Bovine Heat shock 70 kDa protein 13, HSPA13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Heat shock 70 kDa protein 13, HSPA13 ELISA KIT allows for the in vitro quantitative determination of Bovine Heat shock 70 kDa protein 13, HSPA13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Heat shock 70 kDa protein 13, HSPA13 ELISA KIT has been pre-coated with an Bovine Heat shock 70 kDa protein 13, HSPA13 antibody specific to Bovine Heat shock 70 kDa protein 13, HSPA13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Heat shock 70 kDa protein 13, HSPA13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Heat shock 70 kDa protein 13, HSPA13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Heat shock 70 kDa protein 13, HSPA13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!