Bovine Hepatic triacylglycerol lipase, LIPC ELISA KIT
Product Name:Bovine Hepatic triacylglycerol lipase, LIPC ELISA KIT
Packing:96T
Catalog No.:ELI-02628b
Gene Name:Bovine Hepatic triacylglycerol lipase, LIPC
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Hepatic triacylglycerol lipase, LIPC
Alternative Name:LIPC,Bovine Hepatic triacylglycerol lipase, LIPC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Hepatic triacylglycerol lipase, LIPC ELISA KIT allows for the in vitro quantitative determination of Bovine Hepatic triacylglycerol lipase, LIPC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Hepatic triacylglycerol lipase, LIPC ELISA KIT has been pre-coated with an Bovine Hepatic triacylglycerol lipase, LIPC antibody specific to Bovine Hepatic triacylglycerol lipase, LIPC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Hepatic triacylglycerol lipase, LIPC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Hepatic triacylglycerol lipase, LIPC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Hepatic triacylglycerol lipase, LIPC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!