Bovine IgA- inducing protein, IGIP ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

IGIP,C5orf53; IgA-inducing protein homolog; IgA-inducing protein homolog,IgA-inducing protein

Search name

Bovine IGIP ELISA KIT ,Bovine C5orf53 ELISA KIT ,Bovine IgA-inducing protein homolog ELISA KIT ,Bovine IgA-inducing protein homolog ELISA KIT ,Bovine IgA-inducing protein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine IgA-inducing protein homolog concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IgA-inducing protein homolog, During the reaction, IgA-inducing protein homolog in the sample or standard competes with a fixed amount of biotin-labeled IgA-inducing protein homolog for sites on a pre-coated Monoclonal antibody specific to IgA-inducing protein homolog. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IgA-inducing protein homolog in the samples is then determined by comparing the O.D. of the samples to the standard curve.