Bovine Inter- alpha- trypsin inhibitor heavy chain H4, ITIH4 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Inter-alpha-trypsin inhibitor heavy chain H4,ITI heavy chain H4,ITI-HC4,Inter-alpha-inhibitor heavy chain 4,Inter-alpha-trypsin inhibitor family heavy chain-related protein,IHRP,Plasma kallikrein sensitive glycoprotein 120,Gp120,PK-120, Major acute phase protein,MAP, ITIH4,GP120; H4P; IHRP; ITI-HC4; ITIHL1; PK-120; PK120; ITI heavy chain H4; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); inter-alpha-inhibitor heavy chain 4; inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha-trypsin inhibitor heavy chain H4; inter-alpha-trypsin inhibitor, heavy chain-like 1; plasma kallikrein-sensitive glycoprotein 120; inter-alpha-trypsin inhibitor heavy chain family member 4
Search name
Bovine Inter-alpha-trypsin inhibitor heavy chain H4 ELISA KIT ,Bovine ITI heavy chain H4 ELISA KIT ,Bovine ITI-HC4 ELISA KIT ,Bovine Inter-alpha-inhibitor heavy chain 4 ELISA KIT ,Bovine Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA KIT ,Bovine IHRP ELISA KIT ,Bovine Plasma kallikrein sensitive glycoprotein 120 ELISA KIT ,Bovine Gp120 ELISA KIT ,Bovine PK-120 ELISA KIT ,Bovine Major acute phase protein ELISA KIT ,Bovine MAP ELISA KIT ,Bovine ITIH4 ELISA KIT ,Bovine GP120 ELISA KIT ,Bovine H4P ELISA KIT ,Bovine IHRP ELISA KIT ,Bovine ITI-HC4 ELISA KIT ,Bovine ITIHL1 ELISA KIT ,Bovine PK-120 ELISA KIT ,Bovine PK120 ELISA KIT ,Bovine ITI heavy chain H4 ELISA KIT ,Bovine inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ELISA KIT ,Bovine inter-alpha-inhibitor heavy chain 4 ELISA KIT ,Bovine inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA KIT ,Bovine inter-alpha-trypsin inhibitor heavy chain H4 ELISA KIT ,Bovine inter-alpha-trypsin inhibitor ELISA KIT ,Bovine heavy chain-like 1 ELISA KIT ,Bovine plasma kallikrein-sensitive glycoprotein 120 ELISA KIT ,Bovine inter-alpha-trypsin inhibitor heavy chain family member 4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine inter-alpha-trypsin inhibitor heavy chain h4,Inter-alpha-inhibitor heavy chain 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter-alpha-inhibitor heavy chain 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Inter-alpha-inhibitor heavy chain 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inter-alpha-inhibitor heavy chain 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Inter-alpha-inhibitor heavy chain 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.