Bovine Lactoferrin, LTF ELISA kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Lactotransferrin(Lactoferrin), GIG12; HLF2; growth-inhibiting protein 12; neutrophil lactoferrin; talalactoferrin

Search name
Bovine Lactotransferrin ELISA Kit, Bovine Lactoferrin ELISA Kit, Bovine LTF ELISA Kit, Bovine LF ELISA Kit, Bovine
Talalactoferrin ELISA Kit, Bovine GIG12 ELISA Kit, Bovine growth-inhibiting protein 12 ELISA Kit, Bovine neutrophil lactoferrin
ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine lactotransferrin,Lactoferrin concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactoferrin. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Lactoferrin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Lactoferrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Lactoferrin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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