Bovine Leukotriene E4, LTE4 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Leukotriene E4,LTE4

Search name
Bovine Leukotriene E4 ELISA Kit, Bovine LTE4 ELISA Kit, Leukotriene E4 ELISA Kit, LTE4 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Leukotriene E4,LTE4 concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to LTE4, During the reaction, LTE4 in the sample or standard competes with a fixed amount of
biotin-labeled LTE4 for sites on a pre-coated Monoclonal antibody specific to LTE4. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTE4 in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

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