Bovine Lipoarabinomannan, LAM ELISA Kit
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Bovine LAM ELISA Kit, Bovine Lipoarabinomannan ELISA Kit
Bovine Lipoarabinomannan ELISA KIT allows for the in vitro quantitative determination of Bovine lipoarabinomannan,Lipoarabinomannan concentrations
in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Bovine Lipoarabinomannan ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Lipoarabinomannan, During the reaction, Lipoarabinomannan in the sample or standard competes
with a fixed amount of biotin-labeled Lipoarabinomannan for sites on a pre-coated Monoclonal antibody specific to Lipoarabinomannan.
Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP)
is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Lipoarabinomannan in the samples is then determined by comparing the O.D. of the samples to the
Bovine Lipoarabinomannan ELISA KIT PDF manual download