Bovine Metanephrine, MN ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Metanephrine(MN)

Search name
Bovine Metanephrine ELISA Kit, Bovine MN ELISA Kit,Metanephrine ELISA Kit,MN ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Metanephrine, MN concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to MN, During the reaction, MN in the sample or standard competes with a fixed amount of
biotin-labeled MN for sites on a pre-coated Monoclonal antibody specific to MN. Excess conjugate and unbound sample or standard are
washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a
TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MN in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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