Bovine Neudesin, NENF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Bovine NENF ELISA KIT ,Bovine CIR2 ELISA KIT ,Bovine SCIRP10 ELISA KIT ,Bovine SPUF ELISA KIT ,Bovine SCIRP10-related protein ELISA KIT ,Bovine Spinal cord injury related protein 10 ELISA KIT ,Bovine cell growth-inhibiting protein 47 ELISA KIT ,Bovine cell immortalization-related protein 2 ELISA KIT ,Bovine neuron-derived neurotrophic factor ELISA KIT ,Bovine secreted protein of unknown function ELISA KIT ,Bovine neudesin neurotrophic factor ELISA KIT ,Bovine Neudesin ELISA KIT ,Bovine SPUF protein ELISA KIT
Intended use
Bovine NENF ELISA KIT allows for the in vitro quantitative determination of Bovine Neudesin concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine NENF ELISA KIT has been pre-coated with an antibody specific to Neudesin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forNeudesin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neudesin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neudesin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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