Bovine Neurofilament light polypeptide, NEFL ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Bovine NEFL ELISA KIT ,Bovine Neurofilament light polypeptide ELISA KIT ,Bovine NF-L ELISA KIT ,Bovine CMT1F ELISA KIT ,Bovine CMT2E ELISA KIT ,Bovine NF-L ELISA KIT ,Bovine NF68 ELISA KIT ,Bovine NFL ELISA KIT ,Bovine PPP1R110 ELISA KIT ,Bovine light molecular weight neurofilament protein ELISA KIT ,Bovine neurofilament protein light chain ELISA KIT ,Bovine neurofilament subunit NF-L ELISA KIT ,Bovine neurofilament triplet L protein ELISA KIT ,Bovine neurofilament light polypeptide 68kDa ELISA KIT ,Bovine protein phosphatase 1 regulatory subunit 110 ELISA KIT ,Bovine neurofilament light polypeptide ELISA KIT ,Bovine neurofilament L ELISA KIT ,Bovine 68 kDa neurofilament protein ELISA KIT ,Bovine Neurofilament triplet L protein ELISA KIT
Intended use
Bovine NEFL ELISA KIT allows for the in vitro quantitative determination of bovine neurofilament light polypeptide concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine NEFL ELISA KIT has been pre-coated with an antibody specific to NEFL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NEFL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NEFL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NEFL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine NEFL ELISA KIT Manual Request
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