Bovine Parathyroid hormone- related protein, PTHLH ELISA KIT
Product Name:Bovine Parathyroid hormone- related protein, PTHLH ELISA KIT
Packing:96T
Catalog No.:ELI-02770b
Gene Name:Bovine Parathyroid hormone-related protein, PTHLH
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Parathyroid hormone-related protein, PTHLH
Alternative Name:PTHLH,Bovine Parathyroid hormone-related protein, PTHLH
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Parathyroid hormone- related protein, PTHLH ELISA KIT allows for the in vitro quantitative determination of Bovine Parathyroid hormone-related protein, PTHLH concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Parathyroid hormone- related protein, PTHLH ELISA KIT has been pre-coated with an Bovine Parathyroid hormone-related protein, PTHLH antibody specific to Bovine Parathyroid hormone-related protein, PTHLH .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Parathyroid hormone-related protein, PTHLH and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Parathyroid hormone-related protein, PTHLH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Parathyroid hormone-related protein, PTHLH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!