BovinePepsin A, PGA ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Pepsin A, PGA5,pepsin A-5; pepsin A; PGA3; PGA4; pepsinogen A5; pepsinogen-5; pepsinogen 5, group I (pepsinogen A)

Search name

BOVINEPepsin A ELISA Kit, BOVINEPepsin ELISA Kit,BOVINEPGA5 ELISA KIT,BOVINEpepsin A-5 ELISA KIT,BOVINEPGA3 ELISA KIT, BOVINEpepsinogen A5 ELISA KIT, BOVINEpepsinogen-5 ELISA KIT, BOVINEpepsinogen 5 ELISA KIT, Pepsin A ELISA Kit, Pepsin ELISA Kit, PGA5 ELISA KIT,pepsin A-5 ELISA KIT, PGA3 ELISA KIT,pepsinogen A5 ELISA KIT,pepsinogen-5 ELISA KIT,pepsinogen 5 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovinepepsin a,Pepsin A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pepsin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.