Bovine Phosphatidylethanolamine, PE ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Phosphatidylethanolamine,;PE

Search name
Bovine PE ELISA Kit, Bovine Phosphatidylethanolamine ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Phosphatidylethanolamine concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the c ompetitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
 
pre-coated with an antibody specific to Phosphatidylethanolamine, During the reaction, Phosphatidylethanolamine
 
in the sample or
 
standard competes with a fixed amount of biotin-labeled Phosphatidylethanolamine for sites on a pre-coated Monoclonal antibody
specific to Phosphatidylethanolamine . Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylethanolamine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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