Bovine Phosphatidylinositol 4- kinase alpha, PI4KA ELISA KIT

Product Name:Bovine Phosphatidylinositol 4- kinase alpha, PI4KA ELISA KIT
Packing:96T

Catalog No.:ELI-16024b

Gene Name:Bovine Phosphatidylinositol 4-kinase alpha, PI4KA

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Bovine Phosphatidylinositol 4-kinase alpha, PI4KA

Alternative Name:PI4KA,Bovine Phosphatidylinositol 4-kinase alpha, PI4KA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Phosphatidylinositol 4- kinase alpha, PI4KA ELISA KIT allows for the in vitro quantitative determination of Bovine Phosphatidylinositol 4-kinase alpha, PI4KA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Phosphatidylinositol 4- kinase alpha, PI4KA ELISA KIT has been pre-coated with an Bovine Phosphatidylinositol 4-kinase alpha, PI4KA antibody specific to Bovine Phosphatidylinositol 4-kinase alpha, PI4KA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Phosphatidylinositol 4-kinase alpha, PI4KA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Phosphatidylinositol 4-kinase alpha, PI4KA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Phosphatidylinositol 4-kinase alpha, PI4KA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!