Bovine PtdIns (4, 5) P2, PIP2 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5)P2, PIP2

Search name
Bovine Phosphatidylinositol 4,5-bisphosphate ELISA Kit, Bovine PtdIns(4,5)P2 ELISA Kit,Bovine PIP2 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Phosphatidylinositol 4,5-bisphosphate concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Phosphatidylinositol 4,5-bisphosphate, During the reaction, Phosphatidylinositol 4,5-bisphosphate
in the sample or standard competes with a fixed amount of biotin-labeled Phosphatidylinositol 4,5-bisphosphate for sites on a pre-coated
Monoclonal antibody specific to Phosphatidylinositol 4,5-bisphosphate. Excess conjugate and unbound sample or standard are washed
from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylinositol
4,5-bisphosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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